Bioreactor (bioreactor) refers to any plant or engineering gear that gives bioactive environment. It is a organic characteristic simulator, such as fermentor, immobilized enzyme or immobilized phone counter, the usage of the organic feature of enzyme or organism.
The key to large-scale industrialization and commercialization of animal mobilephone way of life technological know-how is whether or not we can layout appropriate bioreactors. Because of the brilliant distinction between animal cells and microbial cells, the ordinary microbial reactor is needless to say now not appropriate for large-scale cultivation of animal cells. First of all, we have to fulfill ample oxygen underneath low shear pressure and proper mixing country to supply adequate oxygen for telephone boom and telephone product synthesis.
Then what kinds of bioreactors are usually used in the laboratory? According to the fabric of the reactor, they can be divided into glass tank reactor, stainless metal reactor and disposable reactor.
(1) glass tank reactor
At present, most of the reactors used for the lookup of technique improvement are usually glass tanks. The glass tank bioreactor has the traits of bendy configuration, effective function, easy operation and handy to upgrade. It is the great desire for growing animal mobilephone tradition and microbial fermentation lookup and improvement projects, and can be used in animal cells and coliform rods. Bacteria, yeast, fungi, insect cells and plant telephone culture.
(2) stainless metal reactor
So far, the antibody capsules authorised by using FDA are essentially produced in stainless metal reactors. Because the early antibody manufacturing is especially low, the scale of manufacturing is frequently around ten thousand L. Stainless metal reactor normally includes CIP module, SIP module, storage module and so on. Therefore, pipeline connection is very complex, which requires greater necessities for operators. Once there is pollution, we want to take a look at and sterilize the total system. Meanwhile, the stainless metal reactor is additionally dealing with excessive preliminary gear funding and operation cost.
(3) one time reactor
In latest years, disposable reactors rapidly occupy the market at domestic and overseas with the benefits of no cleaning, no sterilization and bendy operation. Compared with the standard stainless metal reactor, whether or not it is phone growth, antibody manufacturing or antibody quality, the use of a one-time reactor can reap excessive similarity, at the equal time, it can substantially limit manufacturing expenses and shorten manufacturing cycle.
With the fast improvement of telephone line building science and tradition medium improvement technology, mobile density and antibody manufacturing have been radically improved, which poses a extreme task to large-scale telephone culture.
The speedy upward jab of the disposable reactor has delivered a extraordinary influence to the usual stainless metal reactor. Because the disposable reactor reduces CIP, SIP and associated verification work at the identical time, it notably improves operation flexibility and reduces air pollution risk. In the future, it will be viable to substitute the stainless metal reactor and turn out to be the mainstream reactor for the manufacturing of antibody, if it can resolve the trouble of dissolving substance / precipitate and manufacturing scale well. In addition, the most important foundation for the amplification of the reactor is nevertheless based totally on the empirical amplification principle, and there are nevertheless some disputes, such as the leaf tip charge as the suitability of the amplification principle. These elements will absolutely extend the issue and hazard of the industrialization of antibody pills in the future.